Coding
DspB

Part:BBa_K1659200:Design

Designed by: Wei Chung Kong   Group: iGEM15_Oxford   (2015-08-28)

Dispersin B, an antibiofilm enzyme that specifically hydrolyses PGA polymers


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 418


Design Notes and Sources

A Hisx6 tag is added at the C-terminus for ease of protein purification using metal-affinity chromatography.

We obtained the peptide sequence for DspB from Kaplan et al's original publication and converted it into a nucleotide sequence using IDT's Codon Optimizer, optimizing based on E. coli K-12's codon table [1].


References

[1] Kaplan, J.B. et al., 2004. Genes Involved in the Synthesis and Degradation of Matrix Polysaccharide in Actinobacillus actinomycetemcomitans and Actinobacillus pleuropneumoniae Biofilms. Journal of Bacteriology, 186(24), pp.8213–8220.